Jenway 73 series spectrophotometers prescribed to measure aspirin concentration
21 December 2010
Commonly included in chemistry courses to demonstrate the principle of using light to measure concentration, analysis of aspirin tablets can be carried out accurately by two different methods.
A new Jenway Application note describes and compares how to determine the concentration of acetylsalicylic acid in aspirin tablets, using both the UV and visible wavelength capabilities of the 73 series spectrophotometers.Method 1 measures salicylic acid’s absorption maxima at 303nm while Method 2 quantifies a violet coloured complex at 530nm after salicylic acid has been derivatised with Fe(III). Three tablets were tested using each method and the results and standard deviations are given in the Application Note.
Pharmaceutical manufacturers routinely use the acid/base titration method to check the aspirin content of commercial tablets. However, assay results obtained using the two methods for the 73 series spectrophotometer gave values in agreement with the manufacturer’s own test results for the batch of tablets used in the Jenway study. This finding demonstrates the value of UV and visible wavelength spectroscopy for the quantitative determination of aspirin concentration within an educational context.
Ideal for use in education and routine quality control applications, the four spectrophotometers in the 73 series feature a large graphical display incorporated into the lid, thereby minimising the overall footprint and saving bench space. The basic models, 7300 and 7305, offer measurement modes for absorbance, % transmittance and concentration, while models 7310 and 7315 add scanning, kinetics and quantitation modes as well as the ability to save results and methods to USB memory stick. Models 7305 and 7315 have a wavelength range extending into the UV region of the spectrum; bandwidth is a narrow 5nm for all four instruments.
Intuitive, icon-driven software ensures simple navigation and selected measurement modes can be deactivated on the advanced models, if required in teaching laboratories. A full range of active and passive accessories is available for the 73 series, including an adjustable path length cuvette holder and an automated 8-cell changer, enhancing versatility and productivity.
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